Strain Data Sheet

RBRC06185

Information1

Image
BRC No.RBRC06185
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Tg(H1-RNAi:EGFP/CAG-HcRed1)1Osb
Former Common nameFVB-red(RNAi)#1, FVB-red/RNAi Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Daisuke Watanabe, School of Science, Kitasato University. The pGtoR plasmid was injected into the pronuclei of FVB-Invs<inv> Tg(EF1-Invs/EGFP)B27Hmd fertilized eggs. Invs<inv> Tg(EF1-Invs/EGFP)B27Hmd genes were removed. FVB/N background.
Strain descriptionpGtoR shRNA gene transgenic mice.
Colony maintenanceCarrier x Carrier [or Crossing to FVB/NJcl]
ReferencesPLoS One, 9(2):e89652 (2014). doi: 10.1371/journal.pone.0089652. 24586938
Development, 130, 1725-1734 (2003). 12642479
FEBS Lett., 532: 227-230 (2002). 12459495

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPhuman RNA polymerase III promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HcRed1Red Fluorescent Protein (Heteractis crispa)UNHcRed1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNAhuman H1 promoter, CMV IE enhancer, chicken beta-action promoter, rabbit beta-globin genomic DNA, Heteractis crispa HcRed cDNA
Research applicationFluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 130, 1725-1734 (2003). PLoS One, 9(2):e89652 (2014). doi: 10.1371/journal.pone.0089652.
DepositorDaisuke Watanabe (The Kitasato Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data