BRC No.RBRC06164
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Edn2<tm1Ywa>
Former Common nameET2/129, B6-Edn2 KO
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background.
Strain descriptionET-2 (Edn2 / endothelin 2) gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a neomycin resistant gene. Homozygous mutant mice die after birth (3-4 weeks of age).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6J(Crlj)]
ReferencesJ. Clin Invest., 123, 2643-2653 (2013). 23676500

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Edn2endothelin 24Edn2<tm1Ywa>targeted mutation 1, Masashi Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)4GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse RNA polymerase II promoter


Donor DNAmouse RNA polymerase II promoter, E. coli Neomycin resistance gene, bovine growth hormone polyadenylation site, mouse Edn2 genomic DNA
Research applicationDevelopmental Biology Research
Hematological Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin Invest., 123, 2643-2653 (2013).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data