Strain Data Sheet

RBRC06163

Strain Information

Image
BRC No.RBRC06163
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ednrb<tm1Ywa>
Former Common nameETB/129, B6-Ednra KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. 129 and C57BL/6 mixed background.
Strain descriptionETB (Ednra / endothelin receptor type B) gene knockout mice. A 4.2 kb segment containing exon 3 was replaced with a neomycin resistant gene. Homozygous mutant mice die after birth (average age at death of 25 days).
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Hosoda K, Hammer R E, Richardson J A, Baynash A G, Cheung J C, Giaid A, Yanagisawa M
Cell, 79, 1267-1276 (1994). 8001159

Vascular endothelin-B receptor system in vivo plays a favorable inhibitory role in vascular remodeling after injury revealed by endothelin-B receptor-knockout mice.
Murakoshi N, Miyauchi T, Kakinuma Y, Ohuchi T, Goto K, Yanagisawa M, Yamaguchi I
Circulation., 106, 1991-1998 (2002). 12370225

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ednrbendothelin receptor type B14Ednrbtargeted mutation 1, Masashi Yanagisawa
  • ABCD syndrome(MedGEN)

  • Hirschsprung disease, susceptibility to, 2(MedGEN)

  • Waardenburg syndrome type 4A(MedGEN)
    • GHGrowth hormone polyA (Bovine)14GH
  • ABCD syndrome(MedGEN)

  • Hirschsprung disease, susceptibility to, 2(MedGEN)

  • Waardenburg syndrome type 4A(MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse RNA polymerase II promoter
  • ABCD syndrome(MedGEN)

  • Hirschsprung disease, susceptibility to, 2(MedGEN)

  • Waardenburg syndrome type 4A(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal blood gas level(MP:0002329)

  • abnormal choroid pigmentation(MP:0005100)

  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal cochlear nerve morphology(MP:0004716)

  • abnormal colon goblet cell morphology(MP:0013795)
    • more 21 phenotypes
  • abnormal colon morphology(MP:0000495)

  • abnormal feces composition(MP:0003868)

  • abnormal hair follicle melanocyte morphology(MP:0004381)

  • abnormal intestinal mucosa morphology(MP:0000511)

  • abnormal intestine physiology(MP:0010155)

  • abnormal large intestine crypts of Lieberkuhn morphology(MP:0004842)

  • aganglionic megacolon(MP:0002926)

  • cachexia(MP:0005150)

  • decreased acute inflammation(MP:0005087)

  • decreased sensory neuron number(MP:0000966)

  • hypercapnia(MP:0002318)

  • hypoalgesia(MP:0003043)

  • hypoxia(MP:0005039)

  • increased mean systemic arterial blood pressure(MP:0004875)

  • increased melanoma incidence(MP:0010275)

  • intestinal obstruction(MP:0003270)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • sensorineural hearing loss(MP:0004740)

  • variable depigmentation(MP:0010016)

  • white spotting(MP:0002938)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse RNA polymerase II promoter, bovine growth hormone polyadenylation site, mouse Ednrb genomic DNA
    Research applicationDevelopmental Biology Research
    Hematological Research
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 79, 1267-1276 (1994).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data