RBRC06163

Information1

Image
BRC No.RBRC06163
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Ednrb<tm1Ywa>
Former Common nameETB/129, B6-Ednra KO
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strain
Appearance
Strain developmentDeveloped by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. 129 and C57BL/6 mixed background.
Strain descriptionETB (Ednra / endothelin receptor type B) gene knockout mice. A 4.2 kb segment containing exon 3 was replaced with a neomycin resistant gene. Homozygous mutant mice die after birth (average age at death of 25 days).
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesCirculation., 106, 1991-1998 (2002). 12370225
Cell, 79, 1267-1276 (1994). 8001159

Health Report

Examination Date / Room / Rack2015/05/25Room:4-ARack:I
2015/03/23Room:4-ARack:I
2015/01/19Room:4-ARack:I
2014/11/17Room:4-ARack:I
2014/09/16Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ednrbendothelin receptor type B14Ednrb<tm1Ywa>targeted mutation 1, Masashi Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)14GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse RNA polymerase II promoter

Information2

Donor DNAE. coli Neomycin resistance gene, mouse RNA polymerase II promoter, bovine growth hormone polyadenylation site, mouse Ednrb genomic DNA
Research applicationDevelopmental Biology Research
Hematological Research
Mouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 79, 1267-1276 (1994).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
DepositorMasashi Yanagisawa (University of Texas Southwestern Medical Center at Dallas)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data