RBRC06161

Information1

Image
BRC No.RBRC06161
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(3xARE/TK-NRF2/venus)F4Tiw
Former Common nameOKD48-venus, B6-ARE-NRF2/venus Tg#F4
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionKeap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and venus fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by venus.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesFree Radic. Biol. Med., 72, 124-133 (2014). 24746614

Health Report

Examination Date / Room / Rack2015/07/27Room:4-ARack:B
2015/05/25Room:4-ARack:B
2015/03/23Room:4-ARack:B
2015/02/04Room:4-ARack:B
2015/02/02Room:4-ARack:B
2015/01/28Room:4-ARack:B
2015/01/19Room:4-ARack:B
2015/01/19Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
NFE2l2nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l23xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)UNVenus

Information2

Donor DNA3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2(1-433) cDNA, Aequorea victoria venus (GFP) cDNA, FLAG tag, SV40 t intron, SV40 enhancer+ori, SV40 polyA signal, Polyomavirus enhancer+ori
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Free Radic. Biol. Med., 72, 124-133 (2014).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp).
DepositorTakao Iwawaki (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Approval form Form V (English)

BRC mice in Publications

No Data