Strain Data Sheet

RBRC06161

Strain Information

Image
BRC No.RBRC06161
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(3xARE/TK-NRF2/venus)F4Tiw
Former Common nameOKD48-venus, B6-ARE-NRF2/venus Tg#F4
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takao Iwawaki, Advanced Science Institute, RIKEN in 2010. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionKeap1-dependent Oxidative stress Detector transgenic mice, named OKD48 (Keap1-dependent Oxidative stress Detector, No-48). The mice carry human Nrf2 and venus fusion gene under control of 3xARE promoter (oxidative stress inducible promoter). Only cells experiencing oxidative stress can be detected by venus.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Temporal activation of Nrf2 in the penumbra and Nrf2 activator-mediated neuroprotection in ischemia-reperfusion injury.
Takagi T, Kitashoji A, Iwawaki T, Tsuruma K, Shimazawa M, Yoshimura S, Iwama T, Hara H
Free Radic. Biol. Med., 72, 124-133 (2014). 24746614

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG FLAG tag (synthetic)UNFLAG
NFE2l2 nuclear factor, erythroid derived 2, like 2 (human)UNNFE2l2 3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter)
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNA3xARE promoter (mouse anxioxidant/electrophile responsive element, herpes simplex virus TK basal promoter), human Nrf2(1-433) cDNA, Aequorea victoria venus (GFP) cDNA, FLAG tag, SV40 t intron, SV40 enhancer+ori, SV40 polyA signal, Polyomavirus enhancer+ori
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Free Radic. Biol. Med., 72, 124-133 (2014). The RECIPIENT shall use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR (Dr. Takao Iwawaki). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTakao Iwawaki (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data