RBRC06160

Information1

Image
BRC No.RBRC06160
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a10<tm1Csk>
Former Common nameSGLT5 knockout mouse, SGLT5 KO, B6-Slc5a10 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a10 gene by homologous recombination. C57BL/6N background.
Strain descriptionSlc5a10 (SGLT5) gene knockout mice. Part of exon 3 through part of 6 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal renal reabsorption (increased urinary fructose and mannose excretion). Fatty liver is also observed in mice fed a high fructose diet. Viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesPLoS One. 2013;8(2):e56681. 23451068

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Slc5a10solute carrier family 5 (sodium/glucose cotransporter), member 1011Slc5a10<tm1Csk>targeted mutation 1, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Information2

Donor DNAマウス Phosphoglycerate kinase-1遺伝子プロモーター, phage T7 gb2遺伝子プロモーター, 大腸菌 ネオマイシン耐性遺伝子 cDNA, 合成核酸 ポリA付加シグナル
Research application
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data