RBRC06156

Information1

Image
BRC No.RBRC06156
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a4a<tm1Csk>
Former Common nameSGLT3a knockout mouse, SGLT3a KO, B6-Slc5a4a KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a4a gene by homologous recombination. C57BL/6N background.
Strain descriptionSlc5a4a (SGLT3a) gene knockout mice. Exon 1 to 2 and part of exon 3 were replaced with a PGK neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack2020/04/06Room:3-2Rack:A
2020/02/03Room:3-2Rack:A
2019/12/02Room:3-2Rack:A
2019/10/16Room:3-2Rack:A
2019/08/05Room:3-2Rack:A
2019/06/03Room:3-2Rack:A
2019/04/01Room:3-2Rack:A
2019/02/04Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Slc5a4asolute carrier family 5, member 4a10Slc5a4a<tm1Csk>targeted mutation 1, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Information2

Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter (synthetic), E. coli Neomycine resistance gene, (synthetic) polyA signal, mouse Slc5a4a genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data