Strain Data Sheet

RBRC06155

Information1

Image
BRC No.RBRC06155
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.Cg-Tg(CAG-Cre)CZ-M02Osb
Former Common nameCAG-Cre, BALB-CAGCre Tg#2
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Shuhei Sakakibara and Teruhito Yasui, Research Institute for Microbial Diseases, Osaka University. Generated by backcrossing to BALB/cAJcl from B6.Cg-Tg(CAG-Cre)CZ-M02Osb (RBRC01828) over 11 times.
Strain descriptionThese transgenic mice express Cre recombinase gene under the control of CAG promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in all over the body. BALB/c background.
Colony maintenanceCarrier x Noncarrier [BALB/cAJcl]
ReferencesBiochem. Biophys. Res. Commun., 321, 275-279 (2004). 15358172

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcreCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Phage P1 Cre recombinase
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 321, 275-279 (2004). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University. (https://www.ccb.osaka-u.ac.jp/en/)
DepositorTeruhito Yasui (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Joh K, Matsuhisa F, Kitajima S, Nishioka K, Higashimoto K, Yatsuki H, Kono T, Koseki H, Soejima H.
Growing oocyte-specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR.
Epigenetics Chromatin 11(1) 28(2018) 29875017