RBRC06153

Information1

Image
BRC No.RBRC06153
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(TOPGAL) Ctnnb1<Rgsc1880>
Former Common nameTOPGAL;Ctnnb1/C429S (B6), B6-TOPGAL Ctnnb1/C429S
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takuya Murata and Yoichi Gondo, RIKEN BioResource center in 2012. C57BL/6J background.
Strain descriptionIns-TOPGAL and Ctnnb1 C429S mutant mice. Ctnnb1 C429S mutant mice were generated by ENU mutagenesis. C492S homozygous mutant mice are infertile. Ins-TOPGAL transgenic mice are useful for visualizing Wnt signal pathway. The transgene contains the nLacZ gene under the control of a TOPFLASH promoter (6x TCF binding motifs upstream of a TK promoter). The transgene construct was flanked by the insulator core elements.
Colony maintenanceTg: Carrier x Noncarrier [C57BL/6JJcl]; Ctnnb1: Heterozygote x Wild-type [C57BL/6JJcl]
ReferencesSci. Rep., 4, 6959 (2014). 25376241
Nat. Rev. Genet., 9, 803-810 (2008). 18781157

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNATCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYoichi Gondo (RIKEN GSC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Mouse of the Month Nov 2014

BRC mice in Publications

No Data