Strain Data Sheet

RBRC06142

Information1

Image
BRC No.RBRC06142
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Tnfsf11<tm1>
Former Common nameRANKL flox, B6-Tnfsf11 floxed
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Toshio Fumoto and Kyoji Ikeda, National Center for Geriatrics and Gerontology (2009-2013). 129Sv/EvTac derived ES cells were used. Frt flanked neo cassette was removed by crossing with CAG-FLPe (B6). The mutant mice were crossed C57BL/6 OVER 9 times.
Strain descriptionRANKL (Tnfsf11) gene floxed mice. Exons 3 and 4 were flanked by loxP site.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygous mice show no obvious abnormality.
ReferencesJ. Bone Miner. Res. (DOI 10.1002/jbmr.2096) 2013 24014480

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnfsf11tumor necrosis factor (ligand) superfamily, member 1114Tnfsf11<tm1Suna>targeted mutation 1, Sunao Takeshita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Information2

Donor DNABacteriophage P1 loxP sites, Yeast FRT site, mouse RANKL genomic DNA
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Bone. Miner. Res., 29, 830-842 (2014).
DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data