RBRC06095

Information1

Image
BRC No.RBRC06095
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(Ayu21-W203*mERT2)1Card
Former Common nameAyu21-W203-mERT2,315, B6-Ayu21-W203/CreER Gt#315
H-2 Haplotype
ES Cell lineKTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University. KTPU8 ES cells were used. C57BL/6 and CBA mixed.
Strain descriptionAyu21-W203-creER driver mice. The beta-geo gene in the exchangeable gene was replaced with a mERT2.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)3En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(Ayu21-W203*mERT2)1Card3Gt(Ayu21-W203*mERT2)1Card<Gt(Ayu21-W203*mERT2)1Card>gene trap 1, Center for Animal Resources & Development

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1mouse PGK polyA signal3Pgk1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1mouse PGK polyA signal3Pgk1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase3cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequence3lox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence3lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)3nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
pSP73E. coli pSP73 vector sequence3pSP73

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)3puromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistance gene, phage P1 cre recombinase, mouse estrogen receptor, yeast FRT (flipase recombination target) sites, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data