RBRC06054

Information1

Image
BRC No.RBRC06054
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Polk<tm3(mpolk)Csk> Tg(gptdelta)1Nmi
Former Common nameinactivated Polk KI and gpt delta double transgenic mice
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Takehiko Nohmi, National Institute of Health Sciences. Inactivate Polk KI mice were generated by using C57BL/6N derived ES cells. The mutant mice were crossed with the gpt delta Tg mice (C57BL/6J background) (RBRC00158). C57BL/6J and C57BL/6N mixed background.
Strain descriptionInactivate Polk knock-in mice, in which Asp at position 197 and Glu at position 198 of exon 6 were replace with Ala, respectively. This mutant mice also have a gpt delta transgene that serves as a tool for assessing the mutagenicity of environmental chemicals.
Colony maintenanceKI: Heterozygote x Wild-type; Tg: Carrier x Carrier
ReferencesDNA Repair, 24, 113-121(2014). 25303778

Health Report

Examination Date / Room / Rack2020/01/27Room:4-BRack:H
2019/11/25Room:4-BRack:H
2019/09/30Room:4-BRack:H
2019/07/29Room:4-BRack:H
2019/05/27Room:4-BRack:H
2019/03/25Room:4-BRack:H
2019/01/28Room:4-BRack:H
2018/11/26Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Polkpolymerase (DNA directed), kappa13Polk<tm3(mpolk)Csk>targeted mutation 3, Chugai Pharmaceutical Co, Ltd

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Polkpolymerase (DNA directed), kappa13Polk<tm3(mpolk)Csk>targeted mutation 3, Chugai Pharmaceutical Co, Ltd

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
gpt Deltaguanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>transgene insertion 1, Takehito Nohmi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
red/gamlambda phage red/gam17red/gam

Information2

Donor DNAmouse inactivated polymerase k cDNA, mouse inactivated polymerase k genomic DNA, lambda phage red/gam Ori, P1 phage loxP sites, lambda 2001, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
Research applicationCancer Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of the mice with the DEPOSITOR and its co-developer, Chugai Pharmaceutical Co., Ltd. (Contact person: Dr. Akira Takeiri, e-mail, takeiriakr@chugai-pharm.co.jp) and must obtain a prior written consent from them and, if necessary, shall enter into an agreement with them.
DepositorTakehiko Noumi (National Institute of Health Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data