Strain Data Sheet


Strain Information

BRC No.RBRC06047
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hes1<tm1Imayo>
Former Common nameB6-Hes1-floxed ; B6-Hes1 floxed
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.
Strain descriptionHes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3'UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesDevelopment, 135(15):2531-2541 (2008). 18579678

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hes1hairy and enhancer of split 1 (Drosophila)16Hes1<tm1Kag>targeted mutation 1, Ryoichiro Kageyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Ordering Information

Donor DNABacteriophage P1 loxP sites, mouse Hes1 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2008 135(15):2531-41.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: and Ryoichiro Kageyama (E-mail: ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ma Z, Xu J, Wu L, Wang J, Lin Q, Chowdhury FA, Mazumder MHH, Hu G, Li X, Du W.
Hes1 deficiency causes hematopoietic stem cell exhaustion.
Stem Cells 38(6) 756-768(2020) 32129527