RBRC06041

Information1

Image
BRC No.RBRC06041
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Hcn4<tm1(tTA)>/2Kyo
Former Common nameICR-Tet-HCN4#1, ICR-Hcn4/tTA KI#2
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040).
Strain descriptionHcn4 tTA knock-in mice.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR] Homozygous mutant mice may be embryonic lethal.
ReferencesJ. Physiol., 591, 1749-1769 (2013). 23318872

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>targeted mutation 1, Noriyuki NakashimaE. coli tet operator, cytomegalovirus CMV promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHhuman growth hormone gene9GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
URA3Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal9

Information2

Donor DNAhuman growth hormone gene, SV40 PolyA signal, E. coli tet operator, cytomegalovirus CMV promoter, Herpes simplex virus thymidine kinase polyadenylation signal, Herpes simplex virus thymidine kinase promoter, E. coli Neomycin resistance gene, E. coli tetracycline transactivator gene, yeast URA3 gene, Phage P1 loxP
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 591, 1749-1769 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S).
DepositorHarunori Ohmori (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data