RBRC06038

Information1

Image
BRC No.RBRC06038
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1(tTA)>/1Kyo
Former Common nameB6-Tet-HCN4#1, B6-Hcn4/tTA KI#1
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040).
Strain descriptionHcn4 tTA knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc] Homozygous mutant mice may be embryonic lethal.
ReferencesJ. Physiol., 591, 1749-1769 (2013). 23318872

Health Report

Examination Date / Room / Rack2015/02/16Room:3-CRack:I
2014/12/15Room:3-CRack:I
2014/10/20Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>targeted mutation 1, Noriyuki NakashimaE. coli tet operator, cytomegalovirus CMV promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHhuman growth hormone gene9GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
URA3Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal9

Information2

Donor DNAtetracycline transactivator tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), SV40 polyA signal, Phage P1 loxP sites, Herpes simplex virus thymidine kinase promoter, E. coli neomycin resistance gene, Herpes simplex virus thymidine kinase polyA signal, yeast URA3 gene, human growth hormone polyA, tetO5-CMV (E. coli tet operator, cytomegalovirus CMV promoter), mouse Hcn4 genomic DNA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 591, 1749-1769 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S).
DepositorHarunori Ohmori (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data