Strain Data Sheet

RBRC06037

Strain Information

Image
BRC No.RBRC06037
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pou5f1<tm1(Pou5f1/EGFP)Osb>
Former Common nameOct3/4-EGFP knock-in mouse
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda, Hitoshi Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-101 ES cells derived from C57BL/6N were used.
Strain descriptionOct3/4-EGFP knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]Homozygous mutant mice are probably lethal.
References
Identification and characterization of subpopulations in undifferentiated ES cell culture.
Toyooka Y, Shimosato D, Murakami K, Takahashi K, Niwa H
Development, 135, 909-918 (2008). 18263842

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)17EGFP mouse Oct3/4 promoter
IRES internal ribosomal entry site (EMCV)17
Pou5f1
MGI:101893		POU domain, class 5, transcription factor 117Pou5f1<tm1(Pou5f1/EGFP)Osb> targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
puro puromycin acyltransferase (Streptomyces alboniger)17puro

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • embryonic lethality (MP:0008762)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Oct3/4 genomic DNA, Jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Streptomyces alboniger puromycin-resistance gene
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.
    DepositorKazuo Yamagata (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Okamoto K, Fujita H, Okada Y, Shinkai S, Onami S, Abe K, Fujimoto K, Sasaki K, Shioi G, Watanabe TM.
    Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
    EMBO J e112305(2023) 37609947