Strain Data Sheet

RBRC06037

Information1

Image
BRC No.RBRC06037
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pou5f1<tm1(Pou5f1/EGFP)Osb>
Former Common nameOct3/4-EGFP knock-in mouse
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda, Hitoshi Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-101 ES cells derived from C57BL/6N were used.
Strain descriptionOct3/4-EGFP knock-in mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]Homozygous mutant mice are probably lethal.
ReferencesDevelopment, 135, 909-918 ( 2008). 18263842

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pou5f1POU domain, class 5, transcription factor 117Pou5f1<tm1(Pou5f1/EGFP)Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)17EGFPmouse Oct3/4 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)17puro

Information2

Donor DNAmouse Oct3/4 genomic DNA, Streptomyces alboniger puromycin-resistance gene, Jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires)
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.
DepositorKazuo Yamagata (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data