Strain Data Sheet
RBRC06036
Information1 | |
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| Image | |
| BRC No. | RBRC06036 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Gt(ROSA)26Sor<tm1.1(H2B-mCherry)Osb> |
| Former Common name | ROSA26-H2B-mCherry |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used. Frt flanked neo was removed by transient expression of FLP recombinase in fertilized eggs of conditional MethylRO mice. |
| Strain description | ROSA26 H2B-mCherry knock-in mice. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality. |
| References | J. Reprod. Dev., 55, 343-350 (2009). 19305125 Hum. Reprod., 24, 2490-2499 (2009). 19574276 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(H2B-mCherry)Osb>targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherryhuman H2B promoter |
Information2 | |
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| Donor DNA | adenovirus splice acceptor (SA), yeast FRT (flipase recombination target) site (F3 site; synthetic variant 3), human H2B genomic DNA, Discosoma sp. mCherry cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Developmental Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Kazuo Yamagata (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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Takahashi H, Nakashima T, Masuda T, Namba M, Sakamoto S, Yamaguchi K, Horimasu Y, Miyamoto S, Iwamoto H, Fujitaka K, Hamada H, Hattori N. Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity. Stem Cell Res Ther 12(1) 471(2021) 34425896 |