Strain Data Sheet

RBRC06035

Information1

Image
BRC No.RBRC06035
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(H2B-mCherry)Osb>
Former Common nameConditional ROSA26-H2B-mCherry
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used.
Strain descriptionROSA26 HB2-mCherry conditional knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality.
ReferencesJ. Reprod. Dev., 55, 343-350 (2009). 19305125
Hum. Reprod., 24, 2490-2499 (2009). 19574276

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(H2B-mCherry)Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherryhuman H2B promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6

Information2

Donor DNAadenovirus splice acceptor (SA), yeast FRT (flipase recombination target) sites (F3 site; synthetic variant 3), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, human H2B genomic DNA, Discosoma sp. mCherry cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuo Yamagata (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data