RBRC06030

Information1

Image
BRC No.RBRC06030
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Kpna1<tm1.1Yyo>
Former Common nameImpa5flox
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tetsuji Moriyama and Yoshihiro Yoneda, Graduate School of Frontier Biosciences, Osaka University in 2006. A neo cassette was removed by crossing with CAG-FLP mice (B6 background).
Strain descriptionKpna1 (IPOA5, Impoirtin alpha 5) gene floxed mice. Exons 2 and 3 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesFEBS J., 278, 1561-1572 (2011). 21371262

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Kpna1karyopherin (importin) alpha 116Kpna1<tm1.1Yyo>targeted mutation 1.1, Yoshihiro Yoneda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Information2

Donor DNABacteriophage P1 locus of X-over P1 (loxP), Saccharomyces cerevisiae Flippase recognition target (FRT), Mouse Kpna1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS J., 278, 1561-1572 (2011).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and notify him the research content. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR/DEVELOPER as co-authors.
DepositorMasahiro Oka (National Institutes of Biomedical Innovation, Health and Nutrition)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data