Strain Data Sheet

RBRC06030

Strain Information

Image
BRC No.RBRC06030
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Kpna1<tm1.1Yyo>
Former Common nameImpa5flox
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Tetsuji Moriyama and Yoshihiro Yoneda, Graduate School of Frontier Biosciences, Osaka University in 2006. A neo cassette was removed by crossing with CAG-FLP mice (B6 background).
Strain descriptionKpna1 (IPOA5, Impoirtin alpha 5) gene floxed mice. Exons 2 and 3 were flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Targeted disruption of one of the importin alpha family members leads to female functional incompetence in delivery.
Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, Yoneda Y
FEBS J., 278, 1561-1572 (2011). 21371262

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site16Frt
Kpna1karyopherin subunit alpha 116Kpna1targeted mutation 1.1, Yoshihiro Yoneda
loxPphage P1 loxP16loxP
loxPphage P1 loxP16loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 locus of X-over P1 (loxP), Saccharomyces cerevisiae Flippase recognition target (FRT), Mouse Kpna1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS J., 278, 1561-1572 (2011).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and notify him the research content. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR/DEVELOPER as co-authors.
    DepositorMasahiro Oka (National Institutes of Biomedical Innovation, Health and Nutrition)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data