RBRC06025

Information1

Image
BRC No.RBRC06025
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR-Tg(Hes5-Ub-NLS-luc2)1Imayo
Former Common namepHes5-NLS-Ub-luc2 ; Hes5-Ub/NLS/luc2 Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain descriptionHes5 luc trasngenic mice. Nuclear-localized destabilized luciferase (Ub-NLS-luc2) is expressed under the control of Hes5 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
ReferencesScience DOI: 10.1126/science.1242366 24179156

Health Report

Examination Date / Room / Rack2016/09/12Room:2-3Rack:A
2016/07/11Room:2-3Rack:A
2016/05/16Room:2-3Rack:A
2016/03/07Room:2-3Rack:A
2016/01/12Room:2-3Rack:A
2015/11/09Room:2-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
UBmutant ubiquitin (G76V)UNUB

Gene symbolGene nameChr.Allele symbolAllele namePromoter
luc2Photinus pyralis LuciferaseUNluc2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNAmouse Hes5 promoter, human ubiquitin, four copies of nuclear-localization signal from SV40 largeT antigen, optimized firefly luciferase luc2 (synthetic) cDNA, Hes5 cDNA, Hes5 3' UTR
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science DOI: 10.1126/science.1242366In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data