RBRC06008

Information1

Image
BRC No.RBRC06008
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Hes5<tm1(venus)Imayo>
Former Common nameVenus-Hes5 fusion Knock-in ; Hes5/venus KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionVenus/Hes5 knock-in mice. Venus-Hes5 fusion protein is expressed from Hes5 locus.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
ReferencesScience DOI: 10.1126/science.1242366 24179156

Health Report

Examination Date / Room / Rack2015/12/07Room:3-2Rack:A
2015/10/05Room:3-2Rack:A
2015/08/03Room:3-2Rack:A
2015/06/01Room:3-2Rack:A
2015/04/06Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hes5hairy and enhancer of split 5 (Drosophila)4Hes5<tm1(venus)Imayo>targeted mutation 1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)4FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)4Venus

Information2

Donor DNAFLAG tag (synthetic), Jellyfish venus cDNA, Yeast Frt (flippase recombination target) site, mouse Hes5 genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science DOI: 10.1126/science.1242366In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data