RBRC06003

Information1

Image
BRC No.RBRC06003
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-Hes1,EGFP)1Imayo
Former Common nameZ/EG-Hes1 ; CAG-Hes1/GFP Tg#1
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionExpression of Hes1 and EGFP can be induced by CAG promoter after Cre-mediated recombination. The transgene was induced to ES cells by electroporation, and the clone having a single transgene copy was used for producing chimera mice.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
null

Information2

Donor DNACAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), Bacteriophage P1 loxP sequence, Neo (E. coli), b-galactosidase (E.coli), mouse Hes1 cDNA, SV40 late polyA signal, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data