RBRC06002

Information1

Image
BRC No.RBRC06002
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo>
Former Common nameRosa26R-Hes1-IRES-EGFP ; ROSA26/Hes1GFP
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionHes1-IRES-EGFP Rosa26 knock-in mice. Expression of Hes1 and EGFP can be induced after Cre-mediated recombination.
Colony maintenanceHomozygote x Homozygote [or Crossing to Slc:ICR]
ReferencesScience DOI: 10.1126/science.1242366 24179156

Health Report

Examination Date / Room / Rack2018/12/17Room:3-DRack:C
2018/10/15Room:3-DRack:C
2018/08/20Room:3-DRack:C
2018/06/18Room:3-DRack:C
2018/04/16Room:3-DRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo>targeted mutation 1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hes1hairy and enhancer of split 1 (Drosophila)6Hes1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SAadenovirus splice acceptor sequence (SA)6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 late polyA signal6

Information2

Donor DNAadenovirus splice acceptor sequence (SA), Bacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), SV40 late polyA signal, mouse Hes1 cDNA, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA,bovine GH polyA, mouse Gt(ROSA)26Sor genomic region
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science DOI: 10.1126/science.1242366In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data