Strain Data Sheet

RBRC05994

Strain Information

Image
BRC No.RBRC05994
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR;D2-Tg(H7-UbLuc-In*del)1Kag
Former Common namepHes7-Ub-luc (intron-less) ; Hes7-luc/dIn Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. The transgene was injected into the pronuclei of DBA/2 fertilized eggs.
Strain descriptionpH7-UbLuc-In (-) (intoronless) transgenic mice. destabilized luciferase (Ub-Luc) is expressed under the control of Hes7 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
References
Intronic delay is essential for oscillatory expression in the segmentation clock.
Takashima Y, Ohtsuka T, Gonzalez A, Miyachi H, Kageyama R
Proc. Natl. Acad. Sci. USA, 108(8):3300-5 (2011). 21300886

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
  • spondylocostal dysostosis 4, autosomal recessive(MedGEN)
    • UBBubiquitin B (human)UNUBB
  • spondylocostal dysostosis 4, autosomal recessive(MedGEN)
    • lucoptimized luciferase (synthetic)UNlucmouse Hes7 promoter, human ubiquitin
  • spondylocostal dysostosis 4, autosomal recessive(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Hes7 promoter, human ubiquitin variant (G76V) cDNA, firefly luciferase (synthetic) cDNA, human influenza virus HA (hemagglutinin) tag sequence
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2011 108(8):3300-5.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data