RBRC05982

Information1

Image
BRC No.RBRC05982
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Hes5<tm1Kag>
Former Common nameHes5 KO (LacZ LacZ knock-in) ; Hes5 KO/lacZ
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2002. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionHes5 gene nls-lacZ targeted reporter mice. The entire coding region was replaced with a IRES-nls-lacZ expression cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
ReferencesJ Neurosci. 2010 30(9):3489-98. 20203209

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hes5hairy and enhancer of split 5 (Drosophila)4Hes5<tm1Kag>targeted mutation 1, Ryoichiro Kageyama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)4nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Apolyadenylic acid additional signal4poly A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)4puromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), beta galactosidase (E. coli), Nuclear-localization signal from SV40 largeT antigen, polyA, Puromycin resistance gene (Streptomyces alboniger), mouse phosphoglycerate kinase promoter (PGK promoter), mouse Hes5 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Neurosci. 2010 30(9):3489-98.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data