BRC No.RBRC05981
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129-Hes5<tm1Fgu>
Former Common nameHes5 KO (Neo) ; Hes5 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Ryoichiro Kageyama, Kyoto University/IGBMC in 1996. R1 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionHes5 gene knockout mice. 724 bp including the coding region from Met1 to Ala76 (bHLH domain) was replaced with a PGK-neo cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
ReferencesEMBO J. 1999 18(8):2196-207. 10205173
Development. 2000 127(11):2323-32. 10804175

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hes5hairy and enhancer of split 5 (Drosophila)4Hes5<tm1Fgu>targeted mutation 1, Francois Guillemot

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), mouse Hes5 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J. 1999 18(8):2196-207.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data