BRC No.RBRC05979
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129-Hes1<tm1Fgu>
Former Common nameHes1 KO ; Hes1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Ryoichiro Kageyama, Kyoto University/IGBMC in 1994. R1 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionHes1 gene knockout mice. The first tree exons were replaced with a PGK-neo cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
ReferencesGenes Dev., 9(24):3136-3148 (1995). 8543157

Health Report

Examination Date / Room / Rack2017/10/16Room:3-BRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hes1hairy and enhancer of split 1 (Drosophila)16Hes1<tm1Fgu>targeted mutation 1, Francois Guillemot

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), mouse Hes1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev. 1995 9(24):3136-48.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data