Strain Data Sheet

RBRC05979

Strain Information

Image
BRC No.RBRC05979
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;129-Hes1<tm1Fgu>
Former Common nameHes1 KO ; Hes1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Ryoichiro Kageyama, Kyoto University/IGBMC in 1994. R1 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionHes1 gene knockout mice. The first tree exons were replaced with a PGK-neo cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
References
Targeted disruption of mammalian hairy and Enhancer of split homolog-1 (HES-1) leads to up-regulation of neural helix-loop-helix factors, premature neurogenesis, and severe neural tube defects.
Ishibashi M, Ang S L, Shiota K, Nakanishi S, Kageyama R, Guillemot F
Genes Dev., 9(24):3136-3148 (1995). 8543157

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hes1hes family bHLH transcription factor 116Hes1targeted mutation 1, Francois Guillemot
neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bile duct development(MP:0002929)

  • abnormal bile duct morphology(MP:0002928)

  • abnormal brain development(MP:0000913)

  • abnormal cell differentiation(MP:0005076)

  • abnormal cochlear hair cell morphology(MP:0002622)
    • more 38 phenotypes
  • abnormal common bile duct morphology(MP:0009495)

  • abnormal cystic duct morphology(MP:0009493)

  • abnormal exocrine pancreas morphology(MP:0005220)

  • abnormal extrahepatic bile duct morphology(MP:0009498)

  • abnormal inner ear vestibule morphology(MP:0000034)

  • abnormal intestine morphology(MP:0000477)

  • abnormal nasal placode morphology(MP:0006292)

  • abnormal neural tube morphology(MP:0002151)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal pancreas development(MP:0003934)

  • abnormal pancreas morphology(MP:0001944)

  • abnormal pituitary gland development(MP:0003816)

  • abnormal retina neuronal layer morphology(MP:0006069)

  • abnormal stomach morphology(MP:0000470)

  • abnormal utricle morphology(MP:0006090)

  • abnormal vestibular saccule morphology(MP:0006089)

  • absent gallbladder(MP:0003250)

  • anencephaly(MP:0001890)

  • decreased body length(MP:0001258)

  • decreased club cell number(MP:0010949)

  • decreased embryo size(MP:0001698)

  • disorganized retina layers(MP:0001328)

  • embryonic lethality during organogenesis, incomplete penetrance(MP:0011108)

  • everted embryonic neuroepithelium(MP:0012686)

  • exencephaly(MP:0000914)

  • incomplete rostral neuropore closure(MP:0000928)

  • increased cochlear inner hair cell number(MP:0004395)

  • increased cochlear outer hair cell number(MP:0004401)

  • increased number of pulmonary neuroendocrine bodies(MP:0010919)

  • increased solitary pulmonary neuroendocrine cell number(MP:0010915)

  • increased vestibular hair cell number(MP:0004327)

  • kinked neural tube(MP:0003400)

  • lethality throughout fetal growth and development, incomplete penetrance(MP:0011109)

  • microcephaly(MP:0000433)

  • perinatal lethality, complete penetrance(MP:0011089)

  • pituitary gland hypoplasia(MP:0002659)

  • small lung(MP:0003641)

  • small stomach(MP:0002691)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), mouse Hes1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev. 1995 9(24):3136-48.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data