BRC No.RBRC05977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp14<tm2Ski>
Former Common nameMT1-MMP(MMP14) knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionT1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Cell Sci., 120, 1607-1614 (2007). 17405818
Genes Cells., 14, 617-626 (2009). 19371380

Health Report

Examination Date / Room / Rack2014/03/10Room:2-検Rack:N


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mmp14matrix metallopeptidase 14 (membrane-inserted)14Mmp14<tm2Ski>targeted mutstion 2, Motoharu Seiki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
gptguanine phosphoribosyltransferase14gptmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)14nls


Donor DNAmouse Phosphoglcerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, HSV Thymidine kinase promoter, SV40 nuclear localization signal (NLS), E. coli LacZ, SV40 PolyA signal, E. coli Gpt
Research applicationDermatology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 14, 617-626 (2009).
DepositorMotoharu Seiki (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

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