Strain Data Sheet

RBRC05977

Strain Information

Image
BRC No.RBRC05977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp14<tm2Ski>
Former Common nameMT1-MMP(MMP14) knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionT1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.
Yana I, Sagara H, Takaki S, Takatsu K, Nakamura K, Nakao K, Katsuki M, Taniguchi S, Aoki T, Sato H, Weiss S J, Seiki M
J. Cell Sci., 120, 1607-1614 (2007). 17405818

Cytoplasmic tail of MT1-MMP regulates macrophage motility independently from its protease activity.
Sakamoto T, Seiki M
Genes Cells., 14, 617-626 (2009). 19371380

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mmp14matrix metallopeptidase 14 (membrane-inserted)14Mmp14targeted mutstion 2, Motoharu Seiki
  • Winchester syndrome(MedGEN)
    • gptguanine phosphoribosyltransferase14gptmouse phosphoglycerate kinase promoter (PGK promoter)
  • Winchester syndrome(MedGEN)
    • lacZbeta-galactosidase (E. coli)14
  • Winchester syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)14herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • Winchester syndrome(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)14nls
  • Winchester syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal skeleton development(MP:0002113)

  • abnormal skeleton morphology(MP:0005508)

  • bone fibrous dysplasia(MP:0031348)

  • impaired macrophage chemotaxis(MP:0003799)

  • obsolete no phenotypic analysis(MP:0003012)
    • more 1 phenotypes
  • postnatal lethality(MP:0002082)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 nuclear localization signal (NLS), E. coli LacZ, mouse Phosphoglcerate kinase promoter (PGK promoter), E. coli gpt, HSV Thymidine kinase promoter, E.coli neomycin resistance gene, SV40 PolyA signal
    Research applicationDermatology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 14, 617-626 (2009).
    DepositorMotoharu Seiki (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data