RBRC05973

Information1

Image
BRC No.RBRC05973
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Lrrc36<tm1a(KOMP)Osb>/2F
Former Common nameLrrc36 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain descriptionTex7 gene floxed mice. Exon 3 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]
ReferencesProc. Natl. Acad. Sci. USA, 113, 7704-7710 (2016). 27357688

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lrrc36leucine rich repeat containing 368Lrrc36<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)8En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8human beta-actin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyadenylation site8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyadenylation site8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)UNGH

Information2

Donor DNAEn2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E.coli neomycin phosphotransferase (neoR) gene, yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Lrrc36 genomic DNA, Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal
Research applicationCre/loxP system
FLP/frt system
Reproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data