Strain Data Sheet

RBRC05950

Strain Information

Image
BRC No.RBRC05950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Lrrc32<tm1Hfuj>
Former Common nameGARP-Floxed, B6-Lrrc32 floxed
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii, Research Institute for Microbial Diseases, Osaka University in 2009. iTL BA1 ES Cells derived from (C57BL/6N x 129S6/SvEvTac)F1 were used. The mutant mice were crossed to C57BL/6.
Strain descriptionGARP (Lrrc32) gene floxed mice. Exon 1 of the Lrrc32 gene was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice are viable and fertile.
References
Regulation of the expression of GARP/latent TGF-beta1 complexes on mouse T cells and their role in regulatory T cell and Th17 differentiation.
Edwards J P, Fujii H, Zhou A X, Creemers J, Unutmaz D, Shevach E M
J. Immunol., 190, 5506-5515 (2013). 23645881

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site7Frt
  • cleft palate, proliferative retinopathy, and developmental delay(MedGEN)
    • Lrrc32leucine rich repeat containing 327Lrrc32targeted mutation 1, Hodaka Fujii
  • cleft palate, proliferative retinopathy, and developmental delay(MedGEN)
    • loxPphage P1 loxP7loxP
  • cleft palate, proliferative retinopathy, and developmental delay(MedGEN)
    • loxPphage P1 loxP7loxP
  • cleft palate, proliferative retinopathy, and developmental delay(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal regulatory T cell physiology(MP:0004946)

  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse GARP (LRRC32) genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 5506-5515 (2013).
    DepositorHodaka Fujii (Hirosaki University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data