Strain Data Sheet

RBRC05950

Information1

Image
BRC No.RBRC05950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Lrrc32<tm1Hfuj>
Former Common nameGARP-Floxed, B6-Lrrc32 floxed
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii, Research Institute for Microbial Diseases, Osaka University in 2009. iTL BA1 ES Cells derived from (C57BL/6N x 129S6/SvEvTac)F1 were used. The mutant mice were crossed to C57BL/6.
Strain descriptionGARP (Lrrc32) gene floxed mice. Exon 1 of the Lrrc32 gene was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice are viable and fertile.
ReferencesJ. Immunol., 190, 5506-5515 (2013). 23645881

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lrrc32leucine rich repeat containing 327Lrrc32<tm1Hfuj>targeted mutation 1, Hodaka FujiiGarp

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse GARP (LRRC32) genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 5506-5515 (2013).
DepositorHodaka Fujii (Hirosaki University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data