Strain Data Sheet

RBRC05943

Strain Information

Image
BRC No.RBRC05943
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Mt1-RET)242Ina Tg(Dct-LacZ)A12Jkn
Former Common name242RET;Dct-LacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. Generated by crossing of RET mice and Hr mice (Hos:HRM). The mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionMt1-RET/Dct-lacZ Tg mice. These mice express the lacZ in melanocytes under the control of Dct promoter and the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenanceMt1-RET: Carrier x Noncarrier; Dct-lacZ: Carrier x Carrier (Homozygote x Homozygote)
References
Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Iwamoto T, Takahashi M, Ito M, Hamatani K, Ohbayashi M, Wajjwalku W, Isobe K, Nakashima I
EMBO J., 10, 3167-3175 (1991). 1915289

Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo.
Mackenzie M A, Jordan S A, Budd P S, Jackson I J
Dev. Biol., 192, 99-107(1997). 9405100

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 splicing signal-polyadenylation site, vector sequenceUN
  • oculocutaneous albinism type 8(MedGEN)
    • RETret proto-oncogene (human)UNRETmouse metallothionein-I promoter-enhancer
  • oculocutaneous albinism type 8(MedGEN)
    • lacZbeta-galactosidase (E. coli)UNmouse Dct promoter
  • oculocutaneous albinism type 8(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMus musclus Metallothionein-I promoter-enhancer, Homo sapiens RFP/RET cDNA, SV40 splicing signal-polyadenylation site, vector sequence, Mus musuculus Dct promoter, Escherichia coli LacZ
    Research applicationCancer Research
    Dermatology Research
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasashi Kato (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data