RBRC05930

Information1

Image
BRC No.RBRC05930
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(mCherry-MBD)Osb>
Former Common nameConditional MethylRO (Methylation probe in ROSA26 locus)
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used.
Strain descriptionROSA26 mCherry-MBD conditional knock-in mice (Conditional MethylRO).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality.
ReferencesDev. Biol.,304, 409-419 (2007). 17274975
Dev. Biol.,312, 419-426 (2007). 17964565

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(mCherry-MBD)Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
MBD1methyl-CpG binding domain protein 1 (human)6MBD1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (adenovirus)6SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Information2

Donor DNAadenovirus splice acceptor (SA), yeast FRT (flipase recombination target) site (F3 site; synthetic variant 3), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 polyadenylation sequence, Discosoma sp. mCherry cDNA, human MBD1(methylated DNA binding domain 1) cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCancer Research
Cell Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuo Yamagata (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Category I MTA: FP license of TBUSA

BRC mice in Publications

No Data