Strain Data Sheet

RBRC05930

Strain Information

Image
BRC No.RBRC05930
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(mCherry-MBD)Osb>
Former Common nameConditional MethylRO (Methylation probe in ROSA26 locus)
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used.
Strain descriptionROSA26 mCherry-MBD conditional knock-in mice (Conditional MethylRO).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality.
References
Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.
Yamazaki T, Yamagata K, Baba T
Dev. Biol.,304, 409-419 (2007). 17274975

Centromeric DNA hypomethylation as an epigenetic signature discriminates between germ and somatic cell lineages.
Yamagata K, Yamazaki T, Miki H, Ogonuki N, Inoue K, Ogura A, Baba T
Dev. Biol.,312, 419-426 (2007). 17964565

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA6
Frtyeast FRT (flippase recombination target) site6Frt
Frtyeast FRT (flippase recombination target) site6Frt
GHGrowth hormone polyA (Bovine)6GH
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1, Research Institute for Microbial Diseases, Osaka University
MBD1methyl-CpG binding domain protein 1 (human)6MBD1
SASplice acceptor (adenovirus)6SA
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry
neoneomycin resistance gene (E. coli)6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor (SA), yeast FRT (flipase recombination target) site (F3 site; synthetic variant 3), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 polyadenylation sequence, Discosoma sp. mCherry cDNA, human MBD1(methylated DNA binding domain 1) cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKazuo Yamagata (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data