Strain Data Sheet
RBRC05930
Information1 | |
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| Image | |
| BRC No. | RBRC05930 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Gt(ROSA)26Sor<tm1(mCherry-MBD)Osb> |
| Former Common name | Conditional MethylRO (Methylation probe in ROSA26 locus) |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University in 2012. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used. |
| Strain description | ROSA26 mCherry-MBD conditional knock-in mice (Conditional MethylRO). |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NJcl]Homozygous mutant mice show no obvious abnormality. |
| References | Dev. Biol.,312, 419-426 (2007). 17964565 Dev. Biol.,304, 409-419 (2007). 17274975 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(mCherry-MBD)Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter MBD1methyl-CpG binding domain protein 1 (human)6MBD1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (adenovirus)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Information2 | |
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| Donor DNA | adenovirus splice acceptor (SA), yeast FRT (flipase recombination target) site (F3 site; synthetic variant 3), E. coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 polyadenylation sequence, Discosoma sp. mCherry cDNA, human MBD1(methylated DNA binding domain 1) cDNA, bovine GH polyA, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Cancer Research Cell Biology Research FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Kazuo Yamagata (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |