Strain Data Sheet

RBRC05928

Strain Information

Image
BRC No.RBRC05928
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Map3k5<tm1Hijo> Map3k6<tm1Hijo> Map3k15<tm1Hijo>
Former Common nameASK1/2/3-TKO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. The triple mutant mice were generated by crossing with Map3k5 KO mice, Map3k6 KO mice and Map3k15 KO mice. C57BL/6J background.
Strain descriptionMap3k5 (ASK1) and Map3k15 (ASK3) genes triple knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed). The exons 1 to 5 of Map3k6 were replaced with a NLS-lacZ cassette and a pgk-neo cassette. A part of exon 1 of Map3k15 was replaced with a neo cassette. Triple homozygous mutant mice are viable and fertile.
Colony maintenanceMap3k5: Homozygote x Homozygote; Map3k6: Homozygote x Homozygote; Map3k15: Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]
References
ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis.
Tobiume K, Matsuzawa A, Takahashi T, Nishitoh H, Morita K, Takeda K, Minowa O, Miyazono K, Noda T, Ichijo H
EMBO J., 28, 843-853 (2009). 11266364

Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.
Takeda K, Shimozono R, Noguchi T, Umeda T, Morimoto Y, Naguro I, Tobiume K, Saitoh M, Matsuzawa A, Ichijo H
J. Biol. Chem., 282, 7522-7531 (2007). 17210579

ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Naguro I, Umeda T, Kobayashi Y, Maruyama J, Hattori K, Shimizu Y, Kataoka K, Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
Nat Commun. (2012). 23250415

ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis.
Tobiume K, Matsuzawa A, Takahashi T, Nishitoh H, Morita K, Takeda K, Minowa O, Miyazono K, Noda T, Ichijo H
EMBO rep., 2, 222-228 (2001). 11266364

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)10
GHGrowth hormone polyA (Bovine)10GH
Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5targeted mutation 1, Hidenori Ichijo
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)
GHGrowth hormone polyA (Bovine)4GH
Map3k6mitogen-activated protein kinase kinase kinase 64Map3k6targeted mutation 1, Hidenori Ichijo
lacZbeta-galactosidase (E. coli)4
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)
nlsSV40 large T antigen nuclear localization signal (NLS)4nls
Map3k15mitogen-activated protein kinase kinase kinase 15XMap3k15targeted mutation 1, Hidenori Ichijo
loxPphage P1 loxPXloxP
loxPphage P1 loxPXloxP
neoneomycin resistance gene (E. coli)X

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell physiology(MP:0005621)

  • abnormal kidney physiology(MP:0002136)

  • abnormal response to cardiac infarction(MP:0000343)

  • cardiovascular system phenotype(MP:0005385)

  • decreased keratinocyte apoptosis(MP:0009581)
    • more 10 phenotypes
  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • decreased response of heart to induced stress(MP:0004486)

  • decreased sensitivity to induced cell death(MP:0008944)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hypertension(MP:0000231)

  • increased incidence of tumors by chemical induction(MP:0004499)

  • increased systemic arterial systolic blood pressure(MP:0006144)

  • integument phenotype(MP:0010771)

  • neoplasm(MP:0002006)

  • salt-sensitive hypertension(MP:0004217)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk polyA, Jellyfish (Aequorea victoria) GFP cDNA, Bovine growth hormone PolyA signal, Mouse Map3k5 genomic DNA, SV40 nuclear localization signal sequence, E. coli beta-galactosidase, Mouse Map3k6 genomic DNA, P1 Phage loxP sites, Klebsiella pneumoniae Neomycin resistance gene, Mouse Map3k15 genomic DNA
    Research applicationApoptosis Research
    Cre/loxP system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHidenori Ichijo (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data