Strain Data Sheet
RBRC05928
Information1 | |
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| Image | |
| BRC No. | RBRC05928 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Map3k5<tm1Hijo> Map3k6<tm1Hijo> Map3k15<tm1Hijo> |
| Former Common name | ASK1/2/3-TKO |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. The triple mutant mice were generated by crossing with Map3k5 KO mice, Map3k6 KO mice and Map3k15 KO mice. C57BL/6J background. |
| Strain description | Map3k5 (ASK1) and Map3k15 (ASK3) genes triple knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed). The exons 1 to 5 of Map3k6 were replaced with a NLS-lacZ cassette and a pgk-neo cassette. A part of exon 1 of Map3k15 was replaced with a neo cassette. Triple homozygous mutant mice are viable and fertile. |
| Colony maintenance | Map3k5: Homozygote x Homozygote; Map3k6: Homozygote x Homozygote; Map3k15: Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl] |
| References | EMBO rep., 2, 222-228 (2001). 11266364 EMBO J., 28, 843-853 (2009). 11266364 Nat Commun. (2012). 23250415 J. Biol. Chem., 282, 7522-7531 (2007). 17210579 |
Health Report | |
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| Examination Date / Room / Rack | 2022/05/31Room:4-BRack:F 2022/03/07Room:4-BRack:F 2021/12/06Room:4-BRack:F 2021/09/08Room:4-BRack:F 2021/06/07Room:4-BRack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>targeted mutation 1, Hidenori Ichijo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)10 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)10GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Map3k6mitogen-activated protein kinase kinase kinase 64Map3k6<tm1Hijo>targeted mutation 1, Hidenori Ichijo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)4GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)4nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Map3k15mitogen-activated protein kinase kinase kinase 15XMap3k15<tm1Hijo>targeted mutation 1, Hidenori Ichijo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)X |
Information2 | |
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| Donor DNA | Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk polyA, Jellyfish (Aequorea victoria) GFP cDNA, Bovine growth hormone PolyA signal, Mouse Map3k5 genomic DNA, SV40 nuclear localization signal sequence, E. coli beta-galactosidase, Mouse Map3k6 genomic DNA, P1 Phage loxP sites, Klebsiella pneumoniae Neomycin resistance gene, Mouse Map3k15 genomic DNA |
| Research application | Apoptosis Research Cre/loxP system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hidenori Ichijo (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |