Strain Data Sheet

RBRC05927

Information1

Image
BRC No.RBRC05927
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Map3k5<tm1Hijo> Map3k15<tm1Hijo>
Former Common nameASK1/3-DKO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. The double mutant mice were generated by crossing with Map3k5 KO mice and Map3k15 KO mice. C57BL/6J background.
Strain descriptionMap3k5 (ASK1) and Map3k15 (ASK3) genes double knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed). A part of exon 1 of Map3k15 was replaced with a neo cassette. Double homozygous mutant mice are viable and fertile.
Colony maintenanceMap3k5: Homozygote x Homozygote; Map3k15: Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]
ReferencesEMBO rep., 2, 222-228 (2001). 11266364
Nat Commun. (2012). 23250415

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)10GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k15mitogen-activated protein kinase kinase kinase 15XMap3k15<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)X

Information2

Donor DNAMouse Map3k5 genomic DNA, E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter (PGK promoter), Bovine PolyA signal, Jellyfish (Aequorea victoria) GFP cDNA, Bovine Growth hormone polyA signal, Mouse MAP3K15 genomic DNA, Klebsiella pneumoniae Neomycin resistance gene, P1 Phage loxP
Research applicationApoptosis Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data