Strain Data Sheet

RBRC05924

Strain Information

Image
BRC No.RBRC05924
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Atpif1<tm1>
Former Common nameAtpif1 KO, B6.Cg-Atpif1<tm1>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Masasuke Yoshida, Faculty of Life Sciences, Kyoto Sangyo University in 2010.
Strain descriptionAtpif1 gene knockout mice. Exons 1-3 of the Atpif1 gene was replaced with a neomycin resistant gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygous mutant mice are viable and fertile.
References
IF1, a natural inhibitor of mitochondrial ATP synthase, is not essential for the normal growth and breeding of mice.
Nakamura J, Fujikawa M, Yoshida M
Biosci. Rep., 33 (5) e00067 (2013). 23889209

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atpif1ATPase inhibitory factor 14Atpif1targeted mutation 1, Kyoto Sangyo University Japan
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Atpif1 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biosci. Rep., 33 (5) e00067 (2013). Within a period of 3 years after deposition of the material, user must contact bailor and notify him a content of the research. If the bailor judges that the research should be a collaborative work, then user must include bailor and/or developer's names as co-authors.
    DepositorMasasuke Yoshida (Kyoto Sangyo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data