RBRC05924

Information1

Image
BRC No.RBRC05924
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Atpif1<tm1>
Former Common nameAtpif1 KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Masasuke Yoshida, Faculty of Life Sciences, Kyoto Sangyo University in 2010.
Strain descriptionAtpif1 gene knockout mice. Exons 1-3 of the Atpif1 gene was replaced with a neomycin resistant gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygous mutant mice are viable and fertile.
ReferencesBiosci. Rep., 33 (5) e00067 (2013). 23889209

Health Report

Examination Date / Room / Rack2014/09/16Room:4-ARack:I
2014/07/14Room:4-ARack:I
2014/05/19Room:4-ARack:I
2014/03/24Room:4-ARack:I
2014/01/20Room:4-ARack:I
2013/11/25Room:4-ARack:I
2013/09/24Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Atpif1ATPase inhibitory factor 14Atpif1<tm1Ksuj>targeted mutation 1, Kyoto Sangyo University Japan

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Atpif1 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)(will be anmounced).Within a period of 3 years after deposition of the material, user must contact bailor and notify him a content of the research. If the bailor judges that the research should be a collaborative work, then user must include bailor and/or developer's names as co-authors.
DepositorMasasuke Yoshida (Kyoto Sangyo University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data