Strain Data Sheet

RBRC05923

Strain Information

Image
BRC No.RBRC05923
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Eed<tm1Homy>
Former Common nameEed cKO mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain descriptionEed floxed mice. Exon 6 of the Eed gene was flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
EED mutants impair polycomb repressive complex 2 in myelodysplastic syndrome and related neoplasms.
T Ueda, M Sanada, H Matsui, N Yamasaki, Z-I Honda, L-Y Shih, H Mori, T Inaba, S Ogawa, H Honda
Leukemia, 26, 2557-2560 (2012). 22733077

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Eed
MGI:95286		embryonic ectoderm development7Eed<tm1Homy> targeted mutation 1, Hiroaki Honda
  • Cohen-Gibson syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) site7Frt
    loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse Eed genomic DNA, yeast FRT (flipase recombination target) site, phage P1 loxP
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data