Strain Data Sheet

RBRC05918

Strain Information

Image
BRC No.RBRC05918
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(TOPGAL)
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takuya Murata, RIKEN BioResource center in 2012. The mice were backcrossed to C57BL/6 from TOP-GAL mice of ICR background (RBRC02228).
Strain descriptionIns-TOPGAL transgenic mice for visualizing Wnt signal pathway. The transgene contains the nLacZ gene under the control of a TOPFLASH promoter (6x TCF binding motifs upstream of a TK promoter). The transgene construct was flanked by the insulator core elements.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl] Tg mice are viable and fertile.
References
GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Moriyama A, Kii I, Sunabori T, Kurihara S, Takayama I, Shimazaki M, Tanabe H, Oginuma M, Fukayama M, Matsuzaki Y, Saga Y, Kudo A
Genesis, 45, 90-100 (2007) 17245775

beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y
Sci. Rep., 4, 6959 (2014). 25376241

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNATCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls)
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Genetics Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichi Gondo (RIKEN GSC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Watanabe M, Natsuga K, Nishie W, Kobayashi Y, Donati G, Suzuki S, Fujimura Y, Tsukiyama T, Ujiie H, Shinkuma S, Nakamura H, Murakami M, Ozaki M, Nagayama M, Watt FM, Shimizu H.
    Type XVII collagen coordinates proliferation in the interfollicular epidermis.
    Elife 6 (2017) 28693719