Strain Data Sheet

RBRC05917

Strain Information

Image
BRC No.RBRC05917
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Mir200b<tm1.1Osb> Mir429<T30C>
Former Common namemiR-DKO, Mir200b<tm1.1Osb> Mir429<T30COsb>/39
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Hidetoshi Hasuwa and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain descriptionmiR200b knockout mice having a point mutation in miR429. Homozygous mutant females show a severe infertility due to abnormal ovulation.
Colony maintenanceHeterozygote x Wild-type[C57BL/6NJcl]
References
miR-200b and miR-429 function in mouse ovulation and are essential for female fertility.
Hasuwa H, Ueda J, Ikawa M, Okabe M
Science, 341, 71-73 (2013). 23765281

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mir200bmicroRNA 200b4Mir200btargeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
Mir429microRNA 4294Mir429T30C
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased circulating luteinizing hormone level(MP:0002773)

  • decreased circulating progesterone level(MP:0005185)

  • decreased corpora lutea number(MP:0002680)

  • decreased litter size(MP:0001935)

  • decreased luteinizing hormone level(MP:0005132)
    • more 5 phenotypes
  • decreased oocyte number(MP:0005431)

  • decreased ovulation rate(MP:0003355)

  • prolonged estrus(MP:0009017)

  • reduced female fertility(MP:0001923)

  • reproductive system phenotype(MP:0005389)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse miR-200b-miR-200a-miR-429 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 341, 71-73 (2013).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data