RBRC05911

Information1

Image
BRC No.RBRC05911
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Cd109<tm1Mat>
Former Common nameCD109-KO
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Shinji Mii and Masahide Takahashi, Nagoya University Graduate School of Medicine in 2008. CSL3 ES cells derived from 129S6 were used. The mutant mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionCD109 gene knockout mice. Part of exon 1 and all of exon 2 of the Cd109 gene were replaced with lacZ and PGK-neo cassettes. Homozygous mutant mice show abnormal hair growth, epidermal hyperplasia and thickening, sebaceous gland hyperplasia at P7.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesAm. J. Pathol., 181, 1180-1189 (2012). 22846721
Pathol. Int., 69(5):249-259 (2019). 31219232

Health Report

Examination Date / Room / Rack2014/03/24Room:4-ARack:H
2014/01/29Room:4-ARack:H
2014/01/20Room:4-ARack:H
2014/01/20Room:4-ARack:H
2014/01/20Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cd109CD109 antigen9Cd109<tm1Mat>targeted mutation 1, Masahide Takahashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2human beta-globin polyA9HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)9nls

Information2

Donor DNAnuclear localization signal, E. coli lacZ gene, Mouse phosphoglycerate kinase (PGK) promoter, E. coli neomycin resistance gene, Human beta-globin polyA, Mouse Cd109 genomic DNA
Research applicationCancer Research
Dermatology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 181, 1180-1189 (2012).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasahide Takahashi (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Nov 2013

BRC mice in Publications

No Data