Strain Data Sheet

RBRC05911

Strain Information

Image
BRC No.RBRC05911
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Cd109<tm1Mat>
Former Common nameCD109-KO
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Shinji Mii and Masahide Takahashi, Nagoya University Graduate School of Medicine in 2008. CSL3 ES cells derived from 129S6 were used. The mutant mice were backcrossed to C57BL/6J over 10 times.
Strain descriptionCD109 gene knockout mice. Part of exon 1 and all of exon 2 of the Cd109 gene were replaced with lacZ and PGK-neo cassettes. Homozygous mutant mice show abnormal hair growth, epidermal hyperplasia and thickening, sebaceous gland hyperplasia at P7.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Epidermal hyperplasia and appendage abnormalities in mice lacking CD109.
Mii S, Murakumo Y, Asai N, Jijiwa M, Hagiwara S, Kato T, Asai M, Enomoto A, Ushida K, Sobue S, Ichihara M, Takahashi M
Am. J. Pathol., 181, 1180-1189 (2012). 22846721

CD109: a multifunctional GPI-anchored protein with key roles in tumor progression and physiological homeostasis.
Mii S, Enomoto A, Shiraki Y, Taki T, Murakumo Y, Takahashi M
Pathol. Int., 69(5):249-259 (2019). 31219232

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd109CD109 antigen9Cd109targeted mutation 1, Masahide Takahashi
HBBhuman beta-globin polyA9HBB
lacZbeta-galactosidase (E. coli)9
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)
nlsSV40 large T antigen nuclear localization signal (NLS)9nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal epidermal layer morphology(MP:0001216)

  • abnormal femur morphology(MP:0000559)

  • abnormal hair follicle infundibulum morphology(MP:0010682)

  • abnormal hair growth(MP:0002073)

  • abnormal hair shaft morphology(MP:0003809)
    • more 17 phenotypes
  • abnormal osteoclast differentiation(MP:0008396)

  • abnormal skin adnexa morphology(MP:0010678)

  • decreased bone mineral density of femur(MP:0020010)

  • decreased bone trabecula number(MP:0010869)

  • decreased bone volume(MP:0010876)

  • dilated hair follicle(MP:0010699)

  • epidermal hyperplasia(MP:0001222)

  • increased bone resorption(MP:0004992)

  • increased bone trabecular spacing(MP:0013630)

  • increased circulating alkaline phosphatase level(MP:0002968)

  • osteoporosis(MP:0000066)

  • sebaceous gland hyperplasia(MP:0009794)

  • skin inflammation(MP:0004947)

  • sparse hair(MP:0000416)

  • thick epidermis stratum basale(MP:0009715)

  • thick epidermis suprabasal layer(MP:0009612)

  • thick epidermis(MP:0001219)
    • Detailed phenotype data

    Ordering Information

    Donor DNAnuclear localization signal, E. coli lacZ gene, Mouse phosphoglycerate kinase (PGK) promoter, E. coli neomycin resistance gene, Human beta-globin polyA, Mouse Cd109 genomic DNA
    Research applicationCancer Research
    Dermatology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Am. J. Pathol., 181, 1180-1189 (2012).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasahide Takahashi (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2013

    BRC mice in Publications

    Aono Y, Suzuki Y, Horiguchi R, Inoue Y, Karayama M, Hozumi H, Furuhashi K, Enomoto N, Fujisawa T, Nakamura Y, Inui N, Mii S, Takahashi M, Suda T.
    CD109 on Dendritic Cells Regulates Airway Hyperreactivity and Eosinophilic Airway Inflammation.
    Am J Respir Cell Mol Biol 68(2) 201-212(2023) 36215676