Strain Data Sheet

RBRC05901

Strain Information

Image
BRC No.RBRC05901
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CMV-EGFP/Pkd2l1*del)P
Former Common nameTgPKD2L1deltaP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Manabu Murakami, Faculty of Medicine, University of Miyazaki. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line P (RBRC05901).
Strain descriptionhese transgenic express carry a EGFP and Pkd2l2 variant (lacking the channel pore sequence) fusion gene under the control of the CMV promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6] Tg mice are viable and fertile.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNCMV promoter
Pkd2l1polycystic kidney disease 2-like 1UNPkd2l1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACytomegalovirus CMV promoter, jellyfish EGFP cDNA(enhanced green fluorescent protein), mouse PKD2L1 cDNA, Bovine BGH polyA (bovine growth hormone), E. coli neo
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorManabu Murakami (Hirosaki University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data