Strain Data Sheet

RBRC05896

Strain Information

Image
BRC No.RBRC05896
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Izumo1-Izumo3/mCherry)Osb
Former Common nameIZUMO3-mCherry TG
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo1-Izumo3/mCherry transgenic mice. These transgenic mice express a Izumo3/mCherry protein under the control of the Izumo1 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6D2F1]Tg mice are viable and fertile.
References
IZUMO family member 3, IZUMO3, is involved in male fertility through the acrosome formation.
Inoue N, Satouh Y, Wada I
Mol. Reprod. Dev., (2021). doi: 10.1002/mrd.23520. 34114279

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)UNGH
Izumo3IZUMO family member 3UNIzumo3mouse Izumo1 promoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Izumo3 cDNA, coral mCherry cDNA, mouse Izumo1 promoter, rabbit beta-globin poly (A), bovine growth hormone polyadenylation signal【クラス１】
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Reprod. Dev., (2021). doi: 10.1002/mrd.23520.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data