Strain Data Sheet

RBRC05893

Information1

Image
BRC No.RBRC05893
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(AKT/Ert2,EGFP)1Nkn
Former Common nameAkt-Mer Tg, B6-AKT/Ert2, GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.
Strain descriptionAKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
ReferencesOncogene 26, 4882-4888 (2007). 17297448
Cell Rep. 19;1(4):360-373 (2012). 22832227

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
AKTthymoma viral proto-oncogene (human)UNAKTCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human Estrogen receptor(ERT2) cDNA, Human influenza virus Ha(hemagglutinin) tag, human Akt cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA
Research applicationCancer Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene 26, 4882-4888 (2007). Cell Rep. 19;1(4):360-373 (2012). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data