Strain Data Sheet

RBRC05893

Strain Information

Image
BRC No.RBRC05893
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(AKT/Ert2,EGFP)1Nkn
Former Common nameAkt-Mer Tg, B6-AKT/Ert2, GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.
Strain descriptionAKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]
References
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Murayama K, Kimura T, Tarutani M, Tomooka M, Hayashi R, Okabe M, Nishida K, Itami S, Katayama I, Nakano T
Oncogene 26, 4882-4888 (2007). 17297448

PI3K-Akt-mTORC1-S6K1/2 axis controls Th17 differentiation by regulating Gfi1 expression and nuclear translocation of RORgamma.
Kurebayashi Y, Nagai S, Ikejiri A, Ohtani M, Ichiyama K, Baba Y, Yamada T, Egami S, Hoshii T, Hirao A, Matsuda S, Koyasu S
Cell Rep. 19;1(4):360-373 (2012). 22832227

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
AKTthymoma viral proto-oncogene (human)UNAKTCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
IRESinternal ribosomal entry site (EMCV)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • hair follicle outer root sheath hyperplasia(MP:0010688)

  • ruffled hair(MP:0000420)

  • thick hair follicle outer root sheath(MP:0010690)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human Estrogen receptor(ERT2) cDNA, Human influenza virus Ha(hemagglutinin) tag, human Akt cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA
    Research applicationCancer Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene 26, 4882-4888 (2007). Cell Rep. 19;1(4):360-373 (2012). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data