RBRC05892

Information1

Image
BRC No.RBRC05892
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Reck<tm1Ito>/Noda
Former Common nameRecktm1(neo)Noda, global Reck knockout
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Chiaki Tkahashi, Regina M. Sasahara, Junseo Oh, Kyoto University Graduate School of Medicine, Shigeyoshi Itohara, RIKEN Brain Research Institute. E14 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionReck gene knockout mice. Exon 1 of the Reck gene was replaced with a PGK-neo cassette. Homozygous mutant mice die around E10.5 with defects in collagen fibrils, basal lamina and vascular development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc] Homozygous mutant mice are embryonic lethal.
ReferencesCell, 107, 789-800 (2001). 11747814
Nat. Neurosci., 10, 838-845 (2007). 17558399

Health Report

Examination Date / Room / Rack2013/09/02Room:3-DRack:J
2013/08/26Room:3-DRack:J
2013/08/26Room:3-DRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Reckreversion-inducing-cysteine-rich protein with kazal motifs4Reck<tm1Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Reck genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 107, 789-800 (2001). 1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an
acknowledgment to the DEPOSITOR is requested.
2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
3. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form.
4. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT shall reach agreement on terms and conditions of use of it with DEPOSITOR and shall obtain a prior written consent from the DEPOSITOR.
5. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMakoto Noda (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data