Strain Data Sheet

RBRC05892

Strain Information

Image
BRC No.RBRC05892
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Reck<tm1Ito>/Noda
Former Common nameRecktm1(neo)Noda, global Reck knockout
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Chiaki Tkahashi, Regina M. Sasahara, Junseo Oh, Kyoto University Graduate School of Medicine, Shigeyoshi Itohara, RIKEN Brain Research Institute. E14 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionReck gene knockout mice. Exon 1 of the Reck gene was replaced with a PGK-neo cassette. Homozygous mutant mice die around E10.5 with defects in collagen fibrils, basal lamina and vascular development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc] Homozygous mutant mice are embryonic lethal.
References
The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis.
Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, Sasahara R M, Nishimura S, Imamura Y, Kitayama H, Alexander D B, Ide C, Horan T P, Arakawa T, Yoshida H, Nishikawa S, Itoh Y, Seiki M, Itohara S, Takahashi C, Noda M
Cell, 107, 789-800 (2001). 11747814

RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity.
Muraguchi T, Takegami Y, Ohtsuka T, Kitajima S, Chandana E P, Omura A, Miki T, Takahashi R, Matsumoto N, Ludwig A, Noda M, Takahashi C
Nat. Neurosci., 10, 838-845 (2007). 17558399

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Reckreversion-inducing-cysteine-rich protein with kazal motifs4Recktargeted mutation 1, Shigeyoshi Itohara
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal angiogenesis(MP:0000260)

  • abnormal basal lamina morphology(MP:0004273)

  • abnormal blood vessel morphology(MP:0001614)

  • abnormal brain vasculature morphology(MP:0004950)

  • abnormal developmental vascular remodeling(MP:0009577)
    • more 12 phenotypes
  • abnormal liver vasculature morphology(MP:0009820)

  • abnormal mesenchyme morphology(MP:0006301)

  • abnormal vascular endothelial cell morphology(MP:0006055)

  • abnormal vitelline vascular remodeling(MP:0004076)

  • decreased embryo size(MP:0001698)

  • decreased fetal size(MP:0004200)

  • dilated vasculature(MP:0004938)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • hemorrhage(MP:0001914)

  • internal hemorrhage(MP:0001634)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • pallor(MP:0003717)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Reck genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell, 107, 789-800 (2001). 1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an
    acknowledgment to the DEPOSITOR is requested.
    2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    3. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form.
    4. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT shall reach agreement on terms and conditions of use of it with DEPOSITOR and shall obtain a prior written consent from the DEPOSITOR.
    5. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMakoto Noda (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data