Strain Data Sheet

RBRC05889

Strain Information

Image
BRC No.RBRC05889
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dpysl2<tm1Gosh>
Former Common nameCRMP2KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Naoya Yamashita, Yokohama City University Graduate School of Medicine, Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2006. A floxed neo cassette remains inserted upstream of exon 13. The mutant mice were backcrossed to C57BL/6 over six times.
Strain descriptionCRMP2 (Dpysl2) Ser522Ala mutation knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References
Phosphorylation of CRMP2 (collapsin response mediator protein 2) is involved in proper dendritic field organization.
Yamashita N, Ohshima T, Nakamura F, Kolattukudy P, Honnorat J, Mikoshiba K, Goshima Y
J. Neurosci., 32, 1360-1365 (2012). 22279220

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dpysl2dihydropyrimidinase-like 214Dpysl2targeted mutation 1, Yoshio Goshima
loxPphage P1 loxP14loxP
loxPphage P1 loxP14loxP
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dendrite morphology(MP:0008143)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse Dpysl2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsUse of the BIOLOGICAL RESOURCE shall be limited to a collaborative research requiring co-authorship of RIKEN CBS Katsuhiko Mikoshiba, Ph.D. (DEPOSITOR). Waseda University Center for Advanced Biomedical Sciences Yoshio Goshima, Ph.D., Yokohama City University Yoshiro Goshima, Ph.D. in publication.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data