Strain Data Sheet

RBRC05887

Strain Information

Image
BRC No.RBRC05887
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129P2-Bri3bp<Gt(RST655)Byg>/Dkit
Former Common nameBNAS1 gene trap mice
H-2 Haplotype
ES Cell lineE14TG2aIV [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Daisuke Kitamura, Tokyo University of Science, Research Institute for Biomedical Sciences in 2005. RST655 gene trapped ES cells were used. The mutant mice were crossed to C57BL/6 or BALB/c over 6 times. C57BL/6 background (RBRC05887).
Strain descriptionBNAS1 (Bri3bp) gene-trap mice. pGTOTMpfs gene- trap vector was inserted in the downstream of exon 1.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc] Homozygous mutant mice are viable and fertile.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bri3bpBri3 binding protein5Bri3bpgene trap RST655, BayGenomics
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2
lacZbeta-galactosidase (E. coli)5
neoneomycin resistance gene (E. coli)5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Bri3bp gene, mouse Engrailed2 gene, E. coli beta-geo (beta-galactosidase-Neomycin-resistance fusion gene), SV40 Polyadenylation signal, E. coli pUC plasmid backbone, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human PLAP(placental alkaline phosphatase) cDNA, rat CD4 transmembrane domain (CD4TM) cDNA
    Research applicationCell Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. After deposition of the BIOLOGICAL RESOURCE, two years are assumed to be a collaborative research with the DEPOSITOR.
    DepositorDaisuke Kitamura (Tokyo University of Science)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data