Strain Data Sheet

RBRC05885

Strain Information

Image
BRC No.RBRC05885
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Vdr<tm1Ska>
Former Common nameB6-Vdr KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Shigeaki Kato, Institute of Molecular and Cellular Biosciences, University of Tokyo. C57BL/6 and CBA mixed.
Strain descriptionVitamin D receptor gene knockout mice. 1.1kb region containing exon 2 of Vdr was replaced with a neomycin resistance cassette. Homozygous mutant mice show vitamin D dependent rickets type II.
Colony maintenanceHeterozygote x Wild-type
References
Vitamin D is an important factor in estrogen biosynthesis of both female and male gonads.
Kinuta K, Tanaka H, Moriwake T, Aya K, Kato S, Seino Y
Endocrinology, 141, 1317-1324 (2000). 10746634

Mice lacking the vitamin D receptor exhibit impaired bone formation, uterine hypoplasia and growth retardation after weaning.
Yoshizawa T, Handa Y, Uematsu Y, Takeda S, Sekine K, Yoshihara Y, Kawakami T, Arioka K, Sato H, Uchiyama Y, Masushige S, Fukamizu A, Matsumoto T, Kato S
Nat. Genet., 16, 391-396 (1997). 9241280

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Vdrvitamin D (1,25-dihydroxyvitamin D3) receptor15Vdrtargeted mutation 1, Shigeaki Kato
  • vitamin D-dependent rickets, type 2A(MedGEN)
    • neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)
  • vitamin D-dependent rickets, type 2A(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell number(MP:0006387)

  • abnormal blood homeostasis(MP:0009642)

  • abnormal bone mineralization(MP:0002896)

  • abnormal bone ossification(MP:0008271)

  • abnormal bone structure(MP:0003795)
    • more 75 phenotypes
  • abnormal chemokine secretion(MP:0008722)

  • abnormal coat/ hair morphology(MP:0000367)

  • abnormal colon morphology(MP:0000495)

  • abnormal dendritic cell differentiation(MP:0008115)

  • abnormal epidermis stratum granulosum morphology(MP:0001239)

  • abnormal estrous cycle(MP:0001927)

  • abnormal fat-soluble vitamin level(MP:0005401)

  • abnormal female reproductive system morphology(MP:0001119)

  • abnormal fertility/fecundity(MP:0002161)

  • abnormal fibula morphology(MP:0002187)

  • abnormal gametogenesis(MP:0001929)

  • abnormal glucose tolerance(MP:0005291)

  • abnormal interleukin-1 secretion(MP:0008650)

  • abnormal large intestine crypts of Lieberkuhn morphology(MP:0004842)

  • abnormal limb bone morphology(MP:0002115)

  • abnormal locomotor activation(MP:0003313)

  • abnormal long bone hypertrophic chondrocyte zone(MP:0000165)

  • abnormal long bone metaphysis morphology(MP:0000133)

  • abnormal long bone morphology(MP:0003723)

  • abnormal macrophage chemotaxis(MP:0010760)

  • abnormal male reproductive system morphology(MP:0001145)

  • abnormal response to new environment(MP:0001413)

  • abnormal skin development(MP:0003941)

  • abnormal spermatogenesis(MP:0001156)

  • abnormal startle reflex(MP:0001486)

  • abnormal tibia morphology(MP:0000558)

  • abnormal trabecular bone morphology(MP:0000130)

  • abnormal vitamin D level(MP:0011228)

  • absent mature ovarian follicles(MP:0001132)

  • alopecia(MP:0000414)

  • asthenozoospermia(MP:0002675)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased bone mineral density(MP:0000063)

  • decreased circulating calcium level(MP:0000195)

  • decreased circulating phosphate level(MP:0000198)

  • decreased compact bone thickness(MP:0000135)

  • decreased hair follicle cell proliferation(MP:0030575)

  • decreased interleukin-6 secretion(MP:0008706)

  • decreased intestinal calcium absorption(MP:0011221)

  • decreased locomotor activity(MP:0001402)

  • decreased prepulse inhibition(MP:0009142)

  • decreased regulatory T cell number(MP:0004974)

  • decreased splenocyte proliferation(MP:0009335)

  • decreased susceptibility to autoimmune diabetes(MP:0004804)

  • decreased urination frequency(MP:0011536)

  • decreased vertical activity(MP:0002757)

  • dermal cyst(MP:0001247)

  • dermatitis(MP:0001194)

  • dilated hair follicle(MP:0010699)

  • dilated seminiferous tubule(MP:0009257)

  • female infertility(MP:0001926)

  • flat face(MP:0012175)

  • impaired coordination(MP:0001405)

  • increased circulating alkaline phosphatase level(MP:0002968)

  • increased circulating follicle stimulating hormone level(MP:0001750)

  • increased circulating luteinizing hormone level(MP:0001751)

  • increased circulating parathyroid hormone level(MP:0002904)

  • increased long bone epiphyseal plate size(MP:0006398)

  • increased osteoblast cell number(MP:0004988)

  • increased susceptibility to autoimmune diabetes(MP:0004803)

  • increased testis weight(MP:0004851)

  • integument phenotype(MP:0010771)

  • male infertility(MP:0001925)

  • oligozoospermia(MP:0002687)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • reduced female fertility(MP:0001923)

  • reduced male fertility(MP:0001922)

  • rickets(MP:0003116)

  • short femur(MP:0003109)

  • short snout(MP:0000445)

  • short stride length(MP:0001407)

  • sparse vibrissae(MP:0001283)

  • uterus hypoplasia(MP:0001121)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase (PGK) genomic DNA, E. coli neo, mouse Vdr genomic DNA
    Research applicationDevelopmental Biology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 16, 391-396 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Shigeaki Kato, uskato0525@gmail.com).
    DepositorKou-ichi Jishage (Chugai Pharmaceutical Co., Ltd.)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data