Strain Data Sheet

RBRC05881

Strain Information

Image
BRC No.RBRC05881
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Phf2<tm1.2Yima>
Former Common namePHF2 flox, B6-Phf2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yosuke Okuno and Dr. Yuuki Imai, Institute of Molecular and Cellular Biosciences, The University of Tokyo in 2010. C57BL/6 and 129 mixed. Frt flanked region was removed by crossing with Actb-FLPe mice (B6 background).
Strain descriptionPhf2 gene floxed mice. Exon 6 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice show no obvious abnormality.
References
Epigenetic regulation of adipogenesis by PHF2 histone demethylase.
Okuno Y, Ohtake F, Igarashi K, Kanno J, Matsumoto T, Takada I, Kato S, Imai Y
Diabetes, 62(5):1426-1434 (2013). 23274892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site13Frt
Phf2PHD finger protein 213Phf2targeted mutation 1, Shigeaki Kato
loxPphage P1 loxP13loxP
loxPphage P1 loxP13loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal adipose tissue development(MP:0011167)

  • decreased body weight(MP:0001262)

  • decreased bone mass(MP:0004016)

  • decreased muscle weight(MP:0004232)

  • decreased white adipose tissue mass(MP:0014146)
    • more 3 phenotypes
  • increased brain weight(MP:0002176)

  • neonatal lethality(MP:0002058)

  • postnatal growth retardation(MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Phf2 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Diabetes. 2012 Dec 28.
    DepositorYuki Imai (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data