RBRC05881

Information1

Image
BRC No.RBRC05881
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Phf2<tm1.2Yima>
Former Common namePHF2 flox, B6-Phf2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yosuke Okuno and Dr. Yuuki Imai, Institute of Molecular and Cellular Biosciences, The University of Tokyo in 2010. C57BL/6 and 129 mixed. Frt flanked region was removed by crossing with Actb-FLPe mice (B6 background).
Strain descriptionPhf2 gene floxed mice. Exon 6 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mice show no obvious abnormality.
ReferencesDiabetes, 62(5):1426-1434 (2013). 23274892

Health Report

Examination Date / Room / Rack2017/03/13Room:2-検Rack:D
2017/01/16Room:2-検Rack:D
2016/11/14Room:2-検Rack:D
2016/09/12Room:2-検Rack:D
2014/12/01Room:3-2Rack:A
2014/10/06Room:3-2Rack:A
2014/08/04Room:3-2Rack:A
2014/06/02Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Phf2PHD finger protein 213Phf2<tm1>targeted mutation 1, Shigeaki Kato

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Information2

Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT site, mouse Phf2 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Diabetes. 2012 Dec 28.
DepositorYuki Imai (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data