Strain Data Sheet

RBRC05879

Strain Information

Image
BRC No.RBRC05879
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Kcnj11<tm1Sse>
Former Common nameKir6.2 KO, B6-Kcnj11 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Dr. Takashi Miki and Susumu Seino, Chiba University Graduate School of Medicine in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J over 5 generations.
Strain descriptionKcnj11 gene knockout mice. An exon of Kcnj11 gene was disrupted by neomycin resistance gene insertion. Homozygous mutant mice are viable and fertile, but show a somewhat low reproductive performance (nurturing) compared to wild-type.
Colony maintenanceHeterozygote, Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
ATP-sensitive K+ channels in the hypothalamus are essential for the maintenance of glucose homeostasis.
Miki T, Liss B, Minami K, Shiuchi T, Saraya A, Kashima Y, Horiuchi M, Ashcroft F, Minokoshi Y, Roeper J, Seino S
Nat. Neurosci., 4, 507-512 (2001). 11319559

Gs/Gq signaling switch in β cells defines incretin effectiveness in diabetes.
Okechi S Oduori, Naoya Murao, Kenju Shimomura, Harumi Takahashi, Quan Zhang, Haiqiang Dou, Shihomi Sakai, Kohtaro Minami, Belen Chanclon, Claudia Guida, Lakshmi Kothegala, Johan Tolö, Yuko Maejima, Norihide Yokoi, Yasuhiro Minami, Takashi Miki, Patrik Rorsman, Susumu Seino
J. Clin. Invest., 130(12):6639-6655 (2020). 33196462

Defective insulin secretion and enhanced insulin action in KATP channel-deficient mice.
Miki T, Nagashima K, Tashiro F, Kotake K, Yoshitomi H, Tamamoto A, Gonoi T, Iwanaga T, Miyazaki J, Seino S
Proc. Natl. Acad. Sci. USA, 95, 10402-10406 (1998). 9724715

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kcnj11potassium inwardly rectifying channel, subfamily J, member 117Kcnj11targeted mutation 1, Susumu Seino
  • diabetes mellitus, permanent neonatal 2(MedGEN)

  • diabetes mellitus, transient neonatal, 3(MedGEN)

  • hyperinsulinemic hypoglycemia, familial, 2(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young type 13(MedGEN)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 2 diabetes mellitus(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)
  • diabetes mellitus, permanent neonatal 2(MedGEN)

  • diabetes mellitus, transient neonatal, 3(MedGEN)

  • hyperinsulinemic hypoglycemia, familial, 2(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young type 13(MedGEN)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 2 diabetes mellitus(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal action potential(MP:0005402)

  • abnormal emotion/affect behavior(MP:0002572)

  • abnormal food intake(MP:0005449)

  • abnormal glucose homeostasis(MP:0002078)

  • abnormal locomotor activation(MP:0003313)
    • more 38 phenotypes
  • abnormal motor capabilities/coordination/movement(MP:0002066)

  • abnormal muscle relaxation(MP:0004036)

  • abnormal nerve fiber response intensity(MP:0003479)

  • abnormal nerve fiber response(MP:0003477)

  • abnormal pancreatic beta cell physiology(MP:0003562)

  • abnormal spatial reference memory(MP:0008414)

  • convulsive seizures(MP:0000947)

  • decreased abdominal fat pad weight(MP:0009287)

  • decreased aerobic running capacity(MP:0003061)

  • decreased anxiety-related response(MP:0001364)

  • decreased circulating insulin level(MP:0002727)

  • decreased exploration in new environment(MP:0001417)

  • decreased glucagon secretion(MP:0002711)

  • decreased insulin secretion(MP:0003059)

  • decreased subcutaneous adipose tissue amount(MP:0008844)

  • decreased susceptibility to diet-induced obesity(MP:0005659)

  • decreased vertical activity(MP:0002757)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hypoglycemia(MP:0000189)

  • impaired coordination(MP:0001405)

  • impaired glucose tolerance(MP:0005293)

  • impaired skeletal muscle contractility(MP:0002841)

  • improved glucose tolerance(MP:0005292)

  • increased anxiety-related response(MP:0001363)

  • increased circulating atrial natriuretic factor(MP:0005522)

  • increased circulating glucose level(MP:0005559)

  • increased circulating insulin level(MP:0002079)

  • increased energy expenditure(MP:0004889)

  • increased insulin secretion(MP:0003058)

  • increased insulin sensitivity(MP:0002891)

  • increased mean systemic arterial blood pressure(MP:0004875)

  • increased muscle fatigability(MP:0003646)

  • increased oxygen consumption(MP:0005289)

  • increased susceptibility to induced morbidity/mortality(MP:0009763)

  • myoclonus(MP:0000243)

  • pigmentation phenotype(MP:0001186)

  • slow postnatal weight gain(MP:0008489)

  • tonic-clonic seizures(MP:0003997)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Kcnj11 genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 95, 10402-10406 (1998).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorSusumu Seino (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data