RBRC05879

Information1

Image
BRC No.RBRC05879
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Kcnj11<tm1Sse>
Former Common nameKir6.2 KO, B6-Kcnj11 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Dr. Takashi Miki and Susumu Seino, Chiba University Graduate School of Medicine in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J over 5 generations.
Strain descriptionKcnj11 gene knockout mice. An exon of Kcnj11 gene was disrupted by neomycin resistance gene insertion. Homozygous mutant mice are viable and fertile, but show a somewhat low reproductive performance (nurturing) compared to wild-type.
Colony maintenanceHeterozygote, Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 95, 10402-10406 (1998). 9724715
Nat. Neurosci., 4, 507-512 (2001). 11319559

Health Report

Examination Date / Room / Rack2015/09/07Room:2-検Rack:D
2013/08/26Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Kcnj11potassium inwardly rectifying channel, subfamily J, member 117Kcnj11<tm1Sse>targeted mutation 1, Susumu Seino

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Kcnj11 genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 1998 95(18):10402-6.The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorSusumu Seino (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data