RBRC05875

Information1

Image
BRC No.RBRC05875
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Lypd4<tm1Osb>/4E
Former Common nameLypd4 KO, Lypd4 KO#4E
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Yoshitaka Fujihara and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain descriptionLypd4 gene knockout mice. Homozyous mutant males are infertile.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:B6D2F1]
ReferencesProc. Natl. Acad. Sci. USA, 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116. 31455729

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lypd4Ly6/Plaur domain containing 47Lypd4<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7

Information2

Donor DNAmouse Lypd4 genomic DNA, E. coli Neomycin resistance gene, yeast FRT (flipase recombination target) sites, Phage P1 loxP【クラス1】(以下「残存有」を交配にて取り除く)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal【クラス1】
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data