RBRC05870

Information1

Image
BRC No.RBRC05870
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pmis2<tm1Osb>
Former Common namePmis2 KO
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain developmentDeveloped by Dr. Ryo Yamaguchi, Dr. Masato Ikawa and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2007.
Strain descriptionPmis2 gene knockout mice. Both exons were replaced with a neo cassette. Homozygous mutant males are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesMol. Biol. Cell, 23, 2671-2679 (2012). 22621904

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
4930479M11RikRIKEN cDNA 4930479M11 gene74930479M11Rik <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Pmis2 genomic DNA, E. coli Neomycin resistance gene 【クラス1】(以下「残存有」を交配にて取り除く)Discosoma sp. DsRed2 cDNA, Su9 signal peptid, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), Jellyfish GFP cDNA, mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal 【クラス1】
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mo.l. Biol. Cell, 23, 2671-2679 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data