RBRC05864

Information1

Image
BRC No.RBRC05864
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Spaca3<tm1Osb>/M4G
Former Common nameSpaca3 KO, Spaca3 KO#M4G
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Yoshitaka Fujihara and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain descriptionSpaca3 gene knockout mice.
Colony maintenanceHomozygote x Homozygote [Jcl:B6D2F1]
ReferencesProc. Natl. Acad. Sci. USA, 113(28), 7704-7710 (2016). 27357688

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spaca3sperm acrosome associated 311Spaca3 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11

Information2

Donor DNAmouse Spaca3 genomic DNA, E. coli Neomycin resistance gene, yeast FRT (flipase recombination target) sites, Phage P1 loxP 【クラス1】
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci, USA, 113(28), 7704-7710 (2016).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data